Salmonella-Escherichia coli/Mammalian-Microsome Reverse Mutation Assay with a Confirmatory Assay with Dimethylamino ethylazide (DMAZ)

摘要

Dimethylamino-2-2ethyl azide (DMAZ, 99.3% pure) was tested for mutagenic potentials with Salmonella typhimurium strains, TA 98, TA 100, TA 1535, TA 1537 and Escherichia coli strain WP2 uvrA by plate incorporation method according to OECD TG 471 in compliance with Good Laboratory Practice. The doses tested with Salmonella tester strains and Escherichia coli up to 5000 micrograms/plate both in the presence and absence of S9 mix. The results showed that DMAZ produced positive response in the TA 100 and TA 1537 , both with and without metabolic activation. No positive response was observed with any of the remaining tester strains with and without activation. The confirmatory assay showed similar results with tester strains with and without activation.