SERDP SEED Project (CS-1161) Final Report: Feasibility Study: Lab-on-a- chip and In Situ Bioassay Techniques for Rapid Resolution of Ion Signatures for Disturbances of Biological Significance in Streams

摘要

This project consisted of three tasks. Results for Tasks 1 and 2 were submitted to the SERDP Project Manager previously as detailed reports. Therefore, the results for these two tasks will only be summarized and drawn from where needed for other sections of this report. For Task 1, we evaluated the potential for advanced in situ measurement of ions (e.g., calcium) and water-quality properties (alkalinity, hardness and conductivity) by use of lab- on-a-chip technology, and derived a path to move forward on further development of advanced in situ monitoring techniques. At the request of SERDP, funding for this task was provided by Oak Ridge National Laboratory (Offices of Life Sciences and Environmental Technologies, and Partnerships and Program Development). Task 2 included a screening survey of the chemical, physical, and biological characteristics of seven sites in three streams on the Fort Hood Military Reservation near Killeen, Texas. This task served the purpose of identifying a location that could provide a range of environmental conditions that will be needed for 'ground-truthing' advanced-monitoring techniques. Task 3 was a detailed literature search for in situ bioassay methods that may be suitable for use in developing and validating the use of advanced-monitoring techniques. For this task, special consideration was given to techniques that would be most pertinent to our efforts. The results of Task 3 are given in detail in this report since they have been reported previously.