Response of Breast Cancer Cells to Hormonal Therapy; Quantitative in Vivo NMR Studies

摘要

Our research is directed at determining the specific metabolic pathways and the individual reactions affected by estrogen and tamoxifen in both estrogen receptor-positive (ER+) and estrogen receptor-negative (ER-) breast cancer cells through the use of novel NMR methodology and quantitative analysis. In this award period, we have quantified the effect of estrogen on metabolic fluxes in ER+ cells. This was accomplished using a comprehensive metabolic model and NMR data from cell extracts. The model was further refined to allow for the incorporation of isotopomer, or multiply-labeled, metabolite data. Microcarrier culture, flask, and roller bottle studies were continued in order to expand the methodology used to quantify breast cancer cell metabolism under varied treatment conditions.