Investigation of the Causes of Breast Cancer at the Cellular Level: Isolation of In Vivo Binding Sites of the Human Origin Recognition Complex

摘要

The coordination between cellular DNA replication and mitosis is critical to ensure controlled cell proliferation and accurate transmission of the genetic information as cells divide -two aspects of cellular life tipically lost in cancer. In order to unravel the molecular mechanisms of human DNA replication in normal and cancer cells, we have started a search for human DNA sequences that serve as replicators, this is, binding sites for human proteins involved in the initiation of DNA replication. Preliminary results are presented.