Hemostatic Function of Apheresis Platelets Stored at 4 deg C and 22 deg C.

摘要

Introduction: Platelet refrigeration decreases the risk of bacterial contamination and may preserve function better than standard-of-care room temperature (RT) storage. Benefits could include lower transfusion-related complications, decreased costs, improved hemostasis in acutely bleeding patients, and extended shelf life. In this study, we compared the effects of 22 deg C and 4 deg C storage on the functional and activation status of apheresis platelets. Methods: Apheresis platelets (n 5 per group) were stored for 5 days at 22 deg C with agitation (RT) versus at 4 deg C with agitation (4 deg C + AG) and without (4 deg C). Measurements included platelet counts, mean platelet volume, blood gas analytes, aggregation response, thromboelastography, thromboxane B2 and soluble CD40 ligand release, activation markers, and microparticle formation. Results: Sample pH levels were within acceptable limits for storage products (pH 6.2 7.4). Platelet glucose metabolism (P < 0.05), aggregation response (adenosine diphosphate: RT 0; 4 deg C + AG 5.0 =/- 0.8; 4 deg C 5.6 +/- 0.9; P < 0.05), and clot strength (maximum amplitude: RT 58 +/- 2; 4 deg C + AG 63 +/- 2; 4 deg C 67 +/- 2; P < 0.05) were better preserved at 4 deg C compared with RT storage. Refrigerated samples were more activated compared with RT (P < 0.05), although thromboxane B2 (P < 0.05) and soluble CD40 ligand release (P < 0.05) were higher at RT. Agitation did not improve the quality of 4 deg C-stored samples. Conclusions: Apheresis platelets stored at 4 deg C maintain more viable metabolic characteristics, are hemostatically more effective, and release fewer proinflammatory mediators than apheresis platelets stored at RT over 5 days. Given the superior bacteriologic safety of refrigerated products, these data suggest that cold-stored platelets may improve outcomes for acutely bleeding patients.