Rapid Parallel Screening for Strain Optimization

摘要

We have identified and characterized a biosensor for auxin and constructed the corresponding biosynthetic cassette. The system is shown in figure 1. Three variants have been made so that we can select on ampicillin, chloramphenicol, or kanamycin. The system also contains a co-transcribed GFP marker for quantitation. After full validation of genotypes we will begin characterizing auxin production and the associated readout provided by the biosensor cassette. Mutagenesis of the host and increased auxin production will follow.