Differential Processing of Cyclin E Variants in Normal vs Tumor Cells and Their Role in Breast Cancer Oncogenesis.

摘要

The major goal of this fellowship is to examine the difference in expression of lower molecular weight isoforms of cyclin E between normal and tumor cells. To this end we had proposed to determine the differences in processing of the full length cyclin E into its LMW forms between normal and tumor cells. As a first step toward fulfillment of this aim we had proposed to repeat transfection of FLAG-tagged cyclin E between normal and tumor cells using a GFP reporter gene transfection. We also had proposed to repeat the transfection in 2 other normal and cell lines. We had further proposed to perform immunoprecipitation study of the truncated FLAG cyclin E on transfected cells and lastly to further clone more truncated cyclin E forms within the proposed region for processing.