Directed Secretion by Bone Cells of a Factor that Attracts Breast Cancer Cells.

摘要

The hFOB osteoblast cell line was cultured in both undifferentiated and differentiated states and tested for the capacity of the cell layers to occlude fluorescent-tagged dextrans of 4-, 20- and 40 kD molecular weight. We found that diffusion of all three dextrans through the undifferentiated osteoblast layer occurred at a rate of 45-70% in two hours whereas diffusion through layers of differentiated cells occurred at a rate of 10% in two hours. Osteonectin, a likely chemoattractant for breast cancer cells, was present in media from the undifferentiated cells. Undifferentiated osteoblasts are potentially a source of chemoattractant for breast cancer cells in vivo.