Metabolic Engineering of Reactive Cell Surfaces for Controlled Cell Adhesion

摘要

The objective is to develop techniques for cell surface modification in order to interface cells with synthetic materials in the context of cell- based biosensors. Our approach uses the cell's metabolic machinery for oligosaccharide biosynthesis to deliver novel chemically reactive functional groups to the cell surface. We developed methods for cell adhesion using ketone- hydrazone condensation reactions and a new reaction we developed called the Staudinger ligation. We identified new metabolic pathways for delivery of reactive functional groups to cell surfaces. These tools were used to attach cells to synthetic surfaces in a robust manner with high resistance to shear stress.