Biochemically Vulnerable Sites for Antifungal Intercession in the Control of Fungal Growth

摘要

In the present grant period DNA array experiments were done in an approach towards identifying target genes that might be transcriptionally regulated by UPC2. Nylon membrane supported, genomic scale arrays were hybridized with radiolabeled cDNA from various strains, which were wild type or mutant with various alleles of UPC2. PDA1 was chosen to normalize the data. While many candidate genes were identified, expression of the genes ERG2, ERG2, ERG11, and ERG25 were selected for additional study. No difference from wild- type levels of ERG2 and ERG3 was apparent except in the upc2-1 mutant.