Functional Detection of p53 Mutations in Achival Prostate Cancer Tissue

摘要

In this research project, we have established a yeast functional assay for the detection of p53 mutations from formalin fixed, paraffin imbedded, archival prostate cancer (CaP) tissue. We have demonstrated that the system is more sensitive than SSCP. We have utilized the system to evaluate the dominant negative gain-of-function effects of p53 mutations found in CaP tissue. We have shown that this method can he used for other tissues such as benign prostatic hypertrophy (BPH), lung cancer, and bladder cancer. This will allow investigators to carry out retrospective studies concerning p53 mutations utilizing paraffin imbedded rather than frozen tissue.