Novel Near Infrared Fluorescent Dyes

摘要

The overall objective of this program is to produce new fluorophores suitable for biosensor signal transduction with excitation wavelengths greater than 665 nm and having electrophilic functionalities for covalent attachment to proteins and nucleic acids. The Phase I effort demonstrated the synthesis of representative fluorophores and characterized their fluorescence emission spectra. The main approach was to synthesize novel styryl dyes with features that result in long wavelength absorbance and emission spectra. The results obtained in the Phase I provide a rationale to achieve specific bathochromic shifts This approach will be implemented in the Phase II effort to develop a series of dyes. To date we have a) demonstrated the feasibility of the proposed synthetic approaches to design long-wavelength styryl dyes, b) have prepared a series of dyes with long wavelength absorption, high quantum yield and large extinction coefficients, c) prepared derivatives of styryl dyes with succinimydyl ester groups, and d) demonstrated the feasibility of using these derivatives for covalent labeling of biological macromolecules.