Patterning Lipid Bilayers on Microfabricated Metal Electrodes

摘要

Lipid molecules were immobilized on surface of photolithographically patterned chromium and titanium. Large unilamellar lipid vesicles were found to bind on the native oxide surface of patterned support metals. Metal evaporation and resist liftoff techniques were used to pattern metal an a hydrophobic polymer surface. Lipids bound on solid substrates provide a biological interface for current measuring electrodes to detect bound cell or biomaterial. This patterning technique provides means to specifically bind lipids and conjugated biomaterials (polyethylene glycol (PEG), biotin, fluorescence dyes, and DNA oligomers) to the electrode surface. This technique may be applied to patterning biomaterial on metal inside thermally bonded microfluidic channels, to form titanium coated biomedical implants, and to create robust lipid-conjugated electrodes for biosensor applications.