4-Aminobiphenyl (4-ABP)-DNA Damage in Breast Tissue and Relationship to p53 Mutation and Polymorphisms of Metabolizing Genes.

摘要

The proposed research project investigates the relationship between environmental exposures and genetic susceptibility factors. This project determined the level of 4-aminobiphenyl(ABP)-DNA adduct levels with immunhistochemical staining in 150 determined by PCR-RFLP. The association between 4-ABP-DNA adduct levels and the carcinogen metabolizing genes CYP1A2, NAT2, NAT1 and GSTP1 were examined. Another aim of this project is to determine if exposure to 4-ABP and its metabolites can cause mutations in the p53 gene. The elucidation of the mutational spectra of pS3 for all 150 samples is currently under investigation.