Optical Patch Clamp. Stage III. Single Channel Activity by Ultra- Resolution Optics

摘要

This award has resulted in 3 major achievements: (1) Second harmonic imaging microscopy (SHIM), a new modality for non-linear optical microscopy, SHIM, has been developed and shown to enable 3D imaging of thick specimens with sub-micrometer resolution. (2) Enhancement of second harmonic generation (SHG) signals by gold nanoparticles has been demonstrated and shows great potential for functional mapping of biological surfaces at the single molecule level. (3) A series of new dyes have been synthesized that have uniquely advantageous properties for both SHG and protein labeling applications.