Diagnosing Breast Cancer Using Protease Fingerprint.

摘要

The utility of using substrate phage display library to study disease-relevant metalloproteinase was further proved by identifying the selective substrates for MMP-l4 (NTl-MNP) . In addition, a different proteomic approach of achieving the original aim of identifying new diagnostic marker for breast cancer was applied and reported in this report. An activity-based probe, FP-biotin, was used to analyze the global activity pattern of a class of disease-relevant enzymes, serine hydrolases, in normal epithelial cells and several breast cancer cells. A novel potential tumor suppressor protein,human carboxylesterase- 2, was identified as the result of the comparison. Initial confirmation of the cell line comparison was done by analyzing the regulation at the transcription and translation level. This result is a good demonstration of the usefulness of applying proteomic approaches to analyze complex biological samples in health and disease states.