Fluid Flow Sensitivity of Bone Cells as a Function of Age

摘要

This is the final report of DAMD-17-91-8509. The project was completed and the original hypothesis that fluid flow sensitivity of bone cells decreases as a function of age was confirmed. Additionaly, several other interesting findings regarding mechanotransduction in bone were discovered. The main findings of the project were that GJIC modulates the PGE2 response of bone cells to oscillating fluid flow. Interestingly, we found that PGE2 release in response to oscillating flow does not appear to involve intracellular calcium. Additionally, we have established that while the Ca(2+) response to fluid flow does decrease as a function of age, GJIC does not. We also demonstrated that a lack of the purinergic receptor P2Y2 is at least partially responsible for the inability of fluid flow to mobilize Ca(2+) in some bone cell lines. In summary, the completion of the project has led to a greater understanding of bone cell mechanotransduction and how it changes as a function of age.