Tracing Substrate Utilization by Specific Marine Sedimentary Microorganisms Using DNA Hybridization-Capture Technology

摘要

Our work has effectively merged in situ measurements of specific microbial metabolisms using radio-labeled growth substrates with 16S rDNA phylogenetic characterization of the microbial community in the same sample. Our group worked with pure cultures of bacteria and geochemically well-characterized systems and made numerous note measurements with radio-labeled substrates. We conversed on a successful molecular method to quantify the incorporation of radio-labeled compounds into DNA by specific groups of bacteria.