Role of Growth-Regulated Oncogene (GRO) Proteins in Human Breast Cancer Growth

摘要

Immunohistochemical (IHC) analysis of CXC cytokines (GRO and IL-8) their receptor, CXC-R2, in human breast cancer (HBC) patient samples; (2) IHC analysis of the IL-1 family of cytokines and receptors (potent inducer/regulator of CXC cytokines) in HBC patient samples; (3) Quantitation and correlation of the CXC and IL-1 families in HBC tissue homogenates with estrogen/progesterone receptor expression; (4) Evaluated in vitro bio activity of the CXC and IL-1 families; (5) Developed retrovector transfected HBC cell strains for in vivo studies; and (6) Developed a xenograft ex ova chick embryo model to evaluated the role of CXC and IL-1 in HBC tumor growth in vivo. Conclusions: (1) The CXC and IL-1 families of cytokines and receptors are present within the HBC tumor microenvironment; (2) There exists significant correlations between the expression of CXC/IL-1 families, estrogen receptor status and disease severity; (3) In vitro, IL-1 is a potent inducer of CXC cytokines; (4) CXC receptors are expressed not only in MBC patient tissue, but also on HBC tumor cell lines (5) CXC cytokines do not appear to be universally mitogenic in HBC cell lines, but more likely regulate activities (proliferation, migration) on specific HBC tumor subpopulations; and (6) A novel chick ex ova xenograft model can be used to evaluate HBC tumor growth and invasiveness.