Formation, Characterization, Protein Resistance, and Reactivity of CI3Si(CH2)11(OCH2CH2)3OH Self-Assembled Monolayers.

摘要

We report a method for generating tri(ethylene glycol)-terminated-n- alkyltrichlorosiloxane monolayers on SiO2 surfaces. These chemisorbed films, with a thickness of (caret)2-3 nm, provide an oligo(ethylene glycol) surface that reduces the nonspecific adsorption of proteins and hydroxyl attachment sites for covalently immobilizing biomolecules to the substrate. These mono- molecular films were formed by adsorbing an acetoxy-tri(ethylene glycol)-terminated n- alkyl- trichlorosilane, CH3(C=O)O(CH2CH2O)3(CH2)11 SiCl3, onto glass and Si/SiO2 substrates, where the terminal acetate provided a protecting group for the hydroxyl functionality during self- assembly of the film. After formation of the monolayer, the acetate functionality was reduced chemically to form films exposing a covalently attached -(OCH2CH2)3OH terminus at a density of (caret)3x10(exp 14) molecules/sq cm. Protein adsorption studies verified that the films exhibited notable resistances against the non-specific adsorption of various proteins. Chemical modification of the -(OCH2CH2)3OH surface with protein. A provided a non-adsorbing surface for selective immobilization of immunoglobulins.