X-Ray Crystallography of Botulinum Neurotoxin Type E and Its Functional Subunits.

摘要

Botulinum Neurotoxin type E was crystallized and an electron density map was analyzed at 3 alpha resolution. A new crystal form was also obtained, and may be helpful in improving the phasing. Co-crystallization experiments with gangliosides have also been undertaken. The structure of a complex between tetanus toxin C-fragment and doxorubicin has been determined, and drug binding occurs at the ganglioside binding site. Structure determination of a complex between tetanus toxin C fragment and the ganglioside GDlb is underway, and data have been collected.