Improving the Efficiency of Enzymatic Baeyer-Villiger Oxidations With Whole Engineered Escherichia coli Cells

摘要

The aim of this work is to develop methodology for conducting whole cell biocatalysis that greatly improves its productivity and therefore overcomes the primary disadvantages associated with this technique: long reaction times and low product titers. The model system used for this work is a recombinant Escherichia coli strain that over expresses the enzyme cyclohexanone monooxygenase (CHMO), originally isolated from Acinetobacter sp. NCIB 9871 (Chen et al., 1999). We believe that the results achieved here may drastically enhance the potential commercial viability of this enzyme as well as many other whole cell biocatalysis applications.