Molecular Basis of Double-Strand DNA Break Repair: Crystal Structure of the RAD52/RPA Complex

摘要

Human Rad52 and RPA are involved in the early steps of double- stranded break repair. The repair of double-stranded breaks in chromosomal DNA is of critical importance for the maintenance of genomic integrity. Rad52 and RPA interact in vivo and in vitro and its interaction is involved in the mechanism of double-strand break repair. In this research, the objective was grouped into four parts: (1) To define the minimum interacting regions on Rad52 and RPA. (2) To identify equilibrium binding conditions that favor the formation of the complex. (3) To develop soluble and active fragments of Rad52 and RPA, each with the particular binding domains. (4) To grow x-ray quality crystals of the soluble active fragments preferably in the complexed form. (5) To solve the crystallographic phase problem and interpret a three dimensional map of the structure. (6) To publish any significant findings.