CDNA Libraries from Microdissected Cells in Pathological Sections

摘要

It is difficult to obtain good quality cDNA from RNA obtained from microdissected cells from pathological sections, particularly from paraffin sections. We are studying ways of isolating extremely small quantities of mRNA and/or 1st strand cDNA with magnetic beads. We are developing techniques for increasing the yield of 1st strand and 2nd strand synthesis reactions. In addition, we are studying 2 methods of amplification: the polymerase chain reaction and amplified RNA methods. Most of our work to date has been carried out with total RNA isolated by traditional methods. In the coming year, we will apply our findings to RNA or 1st strand cDNA obtained from pathological sections.