Cloning of a New Gene/s in Chromosome 17p3.2-p13.1 that Control Apoptosis.

摘要

Loss of genetic material (LOH) in the chromosome 17 p13.2 at the microsatellite marker D17S796 has been identified in atypical ductal hyperplasia and in situ ductal carcinoma of the breast. Our results shown LOH at the same region in MCF-10F cells treated with the chemical carcinogen benz(a)pyrene (BP). Moreover, microcell-mediated transfer of an intact copy of human chromosome 17 inhibited the tumorigenicity of BP1E and PCR-SSCP analyzes showed a restoration of the lost material in BP1E-17-neo. These experiments suggested the presence of a tumor suppressor gene in 17p13.2 near the marker D17S796. We have been able to clone a fragment of the genes that could represent the last exon of a bigger peptide. The presence of a 3' splicing site in the putative introns and the ATTAAA region at the 3'end support this idea. The predicted amino acid sequence does not share significant homology with any known protein supporting the idea that this could be a novel protein. Further experiments will be done in order to clone the full-length cDNA and to study the regulation of the expression of this novel gene.