Flow Cytometer Based Biosensor for In-Field Cell Analysis.

摘要

The overall long-term goal of this research is the development of a self contained, fieldable microfluidic flow cytometer unit for in field cell screening and biological microorgansim population assessment. Development of the flow cytometer unit can provide the instrument platform with which specific target organisms of interest within a mixed population can be detected when a suitable labeling technology is becomes available. Targeted organisms can be those of interest to homeland security 201e.g. toxic phytoplankton species, marine pathogens, etc202 or those species of interest within models of nearshore coastal processes. Miniaturization of flow cytometry technology also allows adaptation of this technology for inclusion in AUVs with subsequent deployment and detection of the targeted organisms under in situ coastal conditions. The fast response nature of this flow cytometer cell screener will provide greater detection and response capabilities as well as, upon AUV deployment, possible support to toxicological assessments in an automated and highly controlled manner, all at a much lower cost than existing available technology.