Laser Capture Microdissection Assisted Identification of Epithelial MicroRNA Expression Signatures for Prognosis of Stage I NSCLC.

摘要

The primary aim of this project is to laser microdissect stage I lung cancer samples, perform microRNA profiling of the epithelial and stromal components and develop component specific signatures of prognosis. This report summarizes the work performed on the project so far. Data analyses indicate that there is a significant difference in the microRNA expression profile of epithelia and stroma in NSCLC tumors and that these profiles have prognostic import with stroma having more prognostic value than epithelia. Also, the fold changes seen along with variability of in-situ hybridization (ISH) measurements do not support pursuing the development of prognostic ISH biomarkers. Based on the data, two microRNAs were chosen for further study. miR-372 overexpression makes cell lines more aggressive with increased migration and invasion. miR-146b was chosen as the stromal miRNA for further study. A no-cost extension was granted to complete these experiments.