Laminin-5 Degradation Due to Mustard in Cultured Normal Human Epidermal Keratinocytes (NHEK); Conference paper

摘要

Laminin-5, a heterotrimer of laminin alpha3, Beta3, and gamma2 chains, is essential for a stable attachment of the epidermis to the dermis. Laminin-5 facilitates the recovery of damaged skin. We studied the effects of both mustards on laminin-5 by Western blotting analysis. We observed that in NHEK, mustards degrade laminin-5. Calmodulin antagonist, W7 or the serine protease inhibitor, TLCK prior to mustard exposure prevented mustard-induced degradation of laminin-5. The results provide important information on the mechanism of laminin-5 degradation by mustards and thus will be useful for developing strategies to prevent skin damage due to mustard exposure.