Patient-Specific B-Cell Antibody Factories to Treat Metastatic Disease.

摘要

Sentinel lymph nodes from breast cancer patients have B-- cell antibody producing colonies which can be selected, cultured, expanded and immortalized to provide B-- cell lines expressing anti--cancer antibodies selective for individual patients or breast cancer subtypes. This proposal is to develop the methodology and application to isolate these anti--B cancer antibody producing B-- cells and effectively immortalize them to produce antibody factories. Antibodies derived from these clones will be used to identify antigen distribution in breast cancers, breast cancer cell lines and other human malignancies. The ultimate goal is to obtain panel of antibodies to human breast cancer antigens designated as such by patient-- specific immune biological responses as opposed to reverse genetic or proteomic methodologies currently being explored. The technological challenges within this proposal will be stepwise overcome with a focus on individual tasks provided in the Statement of Work.