Understanding Endogenous c-Myc Function in Human Breast Cancer Development

摘要

My research is focused on BRCA2, whose mutation has been implicated in the development of breast, ovarian, prostate, pancreatic cancers and Fanconi anemia BRCA2 is an extremely large protein that is challenging% to study. In order to study this important tumor suppressor systematically and comprehensively, I have made a series of rabbit polyclonal antibodies against different parts of human BRCA2. These antibodies have led to the observation that BRCA2 colocalizes with BRCAl to S-phase nuclear foci and ionizing radiation-induced% foci and that BRCA2 possibly localizes to a single large nuclear structure in primary human fibroblasts during G1 phase of the cell cycle when BRCAl expression level is low. Using a double-tagging strategy, I have also purified a BRCA2 C-terminal domain complex and identified a pair of ATPases, Tip48 and Tip49, as BRCA2 interacting proteins.