Design of Functional Protein Materials Self-Assembled on Solid-Phase Surface

摘要

For the construction of intelligent materials using protein molecules, it is essential to assemble protein molecules stably on a solid- phase surface in a highly oriented manner. We have designed novel proteins consist of hydrophobic peptide sequence as a self-assembled structural unit and molecular recognition peptide as a functional unit. These designed proteins were expressed in E. coli and purified with affinity chromatography. The resulting proteins retained their functions on a solid-phase surface and they were applied to biosensing system and cellular engineering.