Targeting Human Breast Cancer Cells that Overexpress HER-2/neu mRNA by an Antisense Iron Responsive Element

摘要

In this project, we attempt to establish the utility of an antisense iron-responsive element (AS-IRE) -mediated gene expression system to targeting HER-2/neu-overexpressing breast cancer cells. During the funding period, we have finished the proposed goal stated in Task 1 by identifying the optimal HER-2/ neu antisense IRE, i.e., AS-IRE4. Moreover, we showed preferential cell killing in HER-2/neu-overexpressing MDA-MB-453 cells using hTERT-AS-IRE4-Bax as opposed to in low HER-2/neu-expressing MDA-MB-468 cells (Task 2).