Phospholipase A2(PLA2) Regulates Neuroexocytosis to Counteract Botulinum Toxin A (BONT/A) Poisoning; Conference paper

摘要

In this study, BoNT/A is one of the most serious biological threats faced by the U. S. military and allied forces. Previously we reported that in nerve growth factor (NGF)-differentiated PC12 cells, arachidonic acid (AA) release is associated with acetylcholine (ACh) release and BoNT/A inhibits both. We report the effect of PLA2 over expression on inhibition of ACh exocytosis due to BoNT/A light chain (LC) in PC12 cells. Over expression of PLA2 alone augmented the stimulated release of ACh and AA. PLA2 over expression also effectively prevented the inhibition of stimulated ACh and AA release due to BoNT/A LC.