Fluoride Ion Regeneration of Sarin (GB) From Minipig Tissue and Fluids Following Whole-Body GB Vapor Exposure; Conference paper

摘要

A method has been developed for the analysis of a Sarin (GB) nerve agent biomarker in the tissue and biological fluids of minipigs that is very sensitive and selective. The GB biomarker was the fluoride ion regenerated GB and was found in minipig samples such as red blood cells, brain, liver, lung, and adipose tissue after whole body inhalation exposure. Regenerated GB in the samples was analyzed by GC-FPD after homogenization, addition of fluoride, pH adjustment and C18 solid-phase extraction. Samples were concentrated by injecting 2-200 uL of extract on a Tenax -TA sorbent tube along with 100 pg of decadeuterated diethyl ethyl phosphonate as the internal standard followed by thermal desorption on to the GC column. The method detection limit was 10.5 pg of agent on column. Highest levels were seen in the lungs (22.8 ng/g) and blood (18.8 ng/g) as expected after an inhalation exposure. Regenerated GB levels in red blood cells were approximately 10 times greater than in the plasma. Outside of the lungs and blood certain sections of the brain had some of the highest level of regenerated GB such as the caudate nucleus (5.46 ng/g). Other significant depots of regenerated GB included saliva (137 ng/g), nasal discharge (72 ng/g), and lachrymal discharge (186 ng/g). Limited amounts of free GB were also isolated from saliva (6.91 ng/g) and nasal discharge (0.65 ng/g).