Forskolin, an Inducer of Camp, Up-Regulates Acetylcholinesterase Expression and Protects Against Organophosphate Exposure in Neuro 2A Cells

摘要

Bioscavenger prophylactic therapy using purified human acetylcholinesterase (AChE) or butylcholinesterase (BChE) is a promising treatment for future protection against chemical warfare agent (CWA) exposure. However, the potential limitations of this approach has led to our investigation of alternative bioscavenger approaches using Forskolin, an inducer of cyclic AMP (cAMP). The optimal dose found for repeated treatment of Forskolin was 12-24 M. Exposure to the organophosphate diisopropylflurophophate (DFP) showed that Forskolin treatment protects the cells from DFP cytotoxicity. These results indicate that transcriptional inducers, such as Forskolin, can sufficiently upregulate AChE production and protect cells against CWA exposure.