Evaluating the Influences of Glycosylation on the Antigenicity and Immunogenicity of Ebola virus Glycoprotein

摘要

All N-linked mutants express when transfected into mammalian cells, as shown by IFA and RIPA. Those containing the C mutation at position 586 (C, CD, ABCD) demonstrate a normal level of GP1 but a reduced amount of GP2 by RIP assay. Vaccination with wild-type GP or with mutants A, B, D and AB protected mice from challenge with mouse-adapted Ebola Zaire. Mutant C and ABCD afforded partial protection in both sets of experiments while CD was completely protective in the first experiment but only partially protective in the second experiment. ELISPOT results showed a decrease in breadth and intensity of T- cell responses from mice vaccinated with mutants C, CD and ABCD. Mutants A, B,D and AB had similar responses to wild type ELISA titers were decreased in mutants C, CD and ABCD compared to wild type and elevated slightly in mutants A, B, and D.