High Throughput Sequencing of Germline and Tumor from Men With Early- Onset Metastatic Prostate Cancer.

摘要

In this project, we proposed to us next generation sequencing approaches to analyze the germline (from blood and/or normal prostate tissue) and prostate cancer tissue from 20 men with early-onset, metastatic prostate cancer in order to identify germline variants that increase the risk of developing clinically significant prostate cancer, as well as novel driving somatic alterations. We have successfully, enrolled 9 men and collected germline DNA on 8 of those men and tumor samples on. Exome-sequencing of germline DNA has been performed and is currently being analyzed for 3 men. In the coming year, we plan to continue enrollment, tissue procurement, and sequencing as well as to begin the analysis of sequencing data and identification of genetic variants of interest. Given the uniqueness of the cohort in this project, we expect that novel driving genes with germline and/or somatic variants will be identified, which can be followed up with future functional studies.