Characterization of the Role of the Cytoplasmic Tail of Prostate Specific Membrane Antigen in its Intracellular Localization and Function

摘要

The goal of this award was to identify cytoplasmic proteins that associate with the cytoplasmic tail of PSMA. Using yeast tow-hybrid screening, we identified that PSMA associates with an actin cross-linking proteins called filamin. We have demonstrated this interaction both in vitro and in vivo. We also established that PSMA is localized to he recycling endosome and that PSMA association with filamin is necessary for PSMA targeting to the recycling endosome. Finally, we also showed that the cytoplasmic tail of PSMA through its association with the act in cross-linking protein modulae the NAALADase activity of this protein. We suggest that the cytoplasmic tail of PSMA plays an important role in the function/s of PSMA.