Genome Wide Examination of Allelic Loss in Lobular and Ductal Breast Cancer

摘要

The purpose of this study is to compare allelic loss throughout the genome in lobular and ductal type breast cancers using the commercially available HuSNP assay. We will evaluate the rate of allelic loss on individual chromosome arms in 60 each of lobular and ductal breast cancers obtained from a population-based study (NCI RO1 CA85913, P.I. Janet Daling). We have validated the prototype HuSNP platform for use in fixed tissue samplesSchubert, 2002(number sign)1827, (2) enrolled and identified subjects for the study, (3) flow cytometry cell sorted breast tumors for the study, and (4) established the new array platform for collection of data from 10,000 SNPs in the Porter lab and the array facility at the FHCRC. Information about morphology-specific traits gained from this study of a large number of lobular cancers will lead to an increased understanding of the biology of distinct subsets of breast cancer. It will provide a basis for future studies designed to stratify patients into prognostic and treatment groups and/or inform the development of targeted therapies for specific tumor types.