Effects of Increasing Doses of Activated Recombinant Factor VII (rFVIIa) on Hemostatic Parameters in Swine.

摘要

This study examined dose-response relationships between activated recombinant factor VII (rFVlla) and I() in vivo haemostasis and (2) in vitro measures of coagulation and platelet functions. Anesthetized swine were used. Ear bleeding time (BT) was measured and blood was sampled following increasing doses of rFVlla (0, 90, 180, 360, and 720 micro(g)/kg; n =6) or saline (n=6). BT was not altered by rFVlla. Prothrombin time (PT) using standard or pig-specific methods was decreased by rFVlla. Activated clotting time (ACT) was decreased by rFVlla. Thromboelastography using collagen (COLL) or pig thromboplastin (p-ThP) as agonist demonstrated shorter reaction times, shortened time to reach maximum velocity of clot formation, and increased alpha-angle in the presence of rFVlla.