Serum DNA Microsatellites as Surrogate Genetic Markers of Breast Cancer Progression

摘要

The main objective of the study is the assessment of microsatellite markers with loss of heterozygosity (LOH) in serum, bone marrow (BM), and primary tumors. In addition the study involves the assessment of circulating tumor cells in blood if they correlate with serum circulating DNA. This is the final progress report of the study. The study was delayed due to logistics of the Office of Regulatory Compliance and Quality, U.S. Army Medical Research and Materiel Command and our Human Subjects IRB. The project has continued on and is now completed. We have successfully demonstrated LOH detection in breast cancer patients serum, bone marrow, and tumors. During the course of the study we have developed an optimal assay using capillary array electrophoresis. The assay is more sensitive than the original gel assay proposed. The study demonstrated LOH in serum was a significant prognostic factor. The main objective of the study is the assessment of microsatellite markers with loss of heterozygosity (LOH) in serum, bone marrow (BM), and primary tumors. In addition the study involves the assessment of circulating tumor cells in blood if they correlate with serum circulating DNA. This is the final progress report of the study. The study was delayed due to logistics of the Office of Regulatory Compliance and Quality, U.S. Army Medical Research and Materiel Command and our Human Subjects IRB. The project has continued on and is now completed. We have successfully demonstrated LOH detection in breast cancer patients serum, bone marrow, and tumors. During the course of the study we have developed an optimal assay using capillary array electrophoresis. The assay is more sensitive than the original gel assay proposed. The study demonstrated LOH in serum was a significant prognostic factor.