New Positron-Emitting Probe for Imaging Cell Proliferation in Breast Cancer

摘要

1-N-Methyl-5-(Beta- D-ribofuranosyl)uracil (1-N-methyl-pseudouridine) will be studied as a new imaging probe for cellular proliferation. The specific aims are: (1) to prepare and test 1-N- (3H)methyl-pseudouridine in vitro in comparison with 2-14C)thymidine, (2) to synthe- size 1- N- (11C)methyl- pseudouridine, and (3) to examine 1-N- (11C)methyl-pseudouridine in vivo using micro-PET imaging. This project is still in the early phase of development, consequently, there are no findings, significant results or conclusions that can be reported at this time. Critical starting material (pseudouridine) has been obtained in order to begin the synthetic and radiochemistry development. In addition, 2'- deoxy-pseudouridine and pseudo-thymidine have been obtained in order to facilitate radiochemistry development for C-11 radiolabeling of 1 -N-methyl-pseudouridine. Some of the pseudothymidine has been custom H-3 labeled which should provide additional biological information for this class of pseudo-nucleosides, and will be compared with that of 1-N- 3H)methyl-pseudouridine in the future.