Low Melatonin Production During Adulthood - Phase 2: Association with Levels of Hydroxyl Radical Scavenging and DNA Damage

摘要

The primary purpose of the study was to develop cross-sectional evidence concerning whether or not lower melatonin production levels are associated with increased oxidative DNA guanine damage. Because the results of this study are supportive, confirmatory studies are warranted, followed by prospective chemoprevention studies of melatonin supplementation. Adjuvant cancer treatment studies have not identified any serious melatonin toxicities. High-performance liquid chromatography-electrospray ionization tandem mass spectrometry (HPLC-ESI MS/MS) was used to simultaneously quantitate urinary levels of 8-hydrodeoxyguanosine (8oxodG), 8-hydroxyguanosine (8oxoGuo), and 8- hydroxyguanine (8oxoGua). Overnight creatinine-adjusted urinary 6- sulphatoxymelatonin (aMT6s) production was assayed in an earlier study. The complete overnight urine samples were properly processed and stored. Fifty-five (55) mother-daughter(s)-father triples of urine samples were available. Fifty- one (51) mothers and 67 daughters were actually assayed. Among the mothers, total overnight 8oxodG was inversely significantly (p<0.05) associated with higher levels of aMT6s. Among the daughters, there were no associations with 8oxodG, 8oxoGua, or 8oxoGuo.