Oral DNA Vaccine Encoding Endoglin Eradicates Breast Tumors by Blocking Their Blood Supply.

摘要

Among novel strategies preventing or slowing breast cancer metastasis are those blocking continuous new blood vessel growth, i.e. angiogenesis in the tumor vasculature mediated by proliferating endothelial cells lining such vessels. These cells overexpress a glycoprotein called endoglin which stimulates such vessels. We successfully constructed and evaluated an oral endoglin-based DNA vaccine and demonstrated its capability to induce a robust CD8(+) T cell response that specifically killed proliferating endothelial cells in the breast tumor vasculature. This, in turn, was shown to markedly decrease tumor angiogenesis resulting in a decisive suppress ion of breast tumor metastases leading to doubling in life-span of successfully vaccinated mice in a prophylactic breast tumor metastasis model.