Results of Topical Myeloperoxidase/Glucose Oxidase/Glucose in the Walker-Mason Burn Model

摘要

One area of research at tile ISR is soft tissue trauma encompassing infection control and wound healing. Treatments are evaluated in vivo using the Walker-Mason model which is a fall thickness thermal injury and infection model. We have identified a novel proprietary topical antimicrobial which may be amenable to treating a variety of wounds. The system consists of an enzyme, myeloperoxidase (MPO) and the requisite substrates which have been optimized for the production of bactericidal reactive oxygen species. Sepsis data is gathered for 21 days and end survivorship of the test article groups are compared to the survivorship of tile untreated group. The survival data is supplemented by monitoring the bacterial infection via bioluminescent imaging by using genetically engineered P. aeruginosa (ATCC 59-1244). The survivorship data is conclusive in stating tile Topical Myeloperoxidase antimicrobial was unsuccessful in recovering an animal from septic infection. The imaging data shows tile movement of bacteria from tile dorsum to the internal cavity and infection of internal organs.