Testing of Intravenous Hemostatic Agents in a Novel Swine Model of Bleeding: Preliminary Results With FXa-PCPs

摘要

Finding an intravenous treatment that could assist endogenous clotting mechanisms is a major mission for military researchers. Herein, we report preliminary data on the efficacy of mixtures of highly purified blood coagulation factors (FVIIa and FXa) and phospholipids vesicles (PCPS) to reduce traumatic bleeding. A novel swine model was developed in which (1) bleeding times (BT) and coagulation function could be ascertained following multiple doses of drug administration; and, (2) an exsanguinating injury could subsequently be performed in the same animal, yielding screening information as to the effects of drug pretreatment of blood loss and survival. FXa-PCPS failed to reduce BT or enhance coagulation function and a dose-dependent hypotensive response. FVIIa-PCPS yielded no data suggestive of the profound potentiation of FVIIa activity by PCPS observed in previous in vitro testing. Further study of these drugs for the use of traumatic hemorrhage is not warranted.