Biofluidic Transport and Molecular Recognition in Polymer Microdevices

摘要

Polymeric microfluidic components were fabricated using living- radical photopolymerization (LRPP), including three-dimensional geometries, modified surfaces by grafting, microporous mixers/filters, micropumps, wires, and movable parts. LRPP enables controlled geometry and surface chemistry for fluid control, biomolecular and cell detection, and particle sorting. Key accomplishments include specific cell adhesion and cytocompatibility demonstrated with grafted surfaces, a fluid-responsive polymer micropump integrated on a device and characterized, a porous polymer plug fabricated within a microfluidic channel and used to mix adjacent streams, analyses and verification of wall effects on particle motion in narrow channels, a polymerizable resistance heater as an electrolysis pump, cell detection with fluorescence when cells adhere to a modified surface, rapid antigen/antibody detection at.