Regulated Ubiquitinylation-Dependent Corepressor and Coactivator Complex Exchange as a Potential Target in Estrogen-Dependent Breast Cancer

摘要

The initial focus of my first year of training has been on getting training in basic molecular biology experimental techniques and bioinformatics analysis of microarray and genome-wide chromatin immunoprecipitation data. Successful initial research results have been accepted for publication. Mutant constructs that block specific phosphorylation sites in TBL1 and TBLR1 has been constructed for microinjection and transfection experiments to test functionality of each phosphorylation sites. Phosphopeptide specific antibodies for TBL1 and TBLR1 have been developed and initial characterization of the antibody has been performed. Performed data analysis of genome-wide chromatin immunoprecipitation experiments with ERalpha, RNA polymerase III and histone modification markers and correlate the binding data with expression profiles upon estrogen stimulation.