Effect of Nanoparticles on Complement System in Cell Culture Model

摘要

The research focused on complement activation by nanoparticles which have been already tested in cell culture by AFRL/HEPB Identification of complement activation pathways was carried out by evaluation of the concentration of pathway specific complement split products generated during human plasma exposure to nanoparticles, Measurements were performed using assay kits supplied by QUIDEL Corporation San Diego California, Different sizes of nanoparticles such as silver (Ag; 151,000 nm) molybdenum (MoO3; 30 150 nm), aluminum (Al; 30 103 nm), iron oxide (Fe3O4; 30, 47 nm) and titanium dioxide (TiO2-40nm) were evaluated for their complement activation potential, The complement activation properties of relatively larger particles of cadmium oxide (CdO; 1 micrometer) manganese oxide (MnO2; 1-2 micrometers), and tungsten (W; 27 micrometers) were assessed, Additionally the effects of nanoparticles coated with lipopolysaccharide on complement activator properties were evaluated.