High-Throughput Detection of Bacillus Anthracis Spores using Peptide- Conjugated Nano/Micro-Beads

摘要

Magnetic bead and Q dots are very attractive materials for detecting B. anthracis spores. Magnetic beads can rapidly isolate B. anthracis spores based on magnetic polarity of the pores, whereas Qdots have ideal characteristics for dense spectral multiplexing, narrow emission range and long lifetime, and can perform simple multiplexed analysis. In this study, they investigated developing a rapid, simple and highly sensitive detection method for detecting B. anthracis spores by using fluorescent nanobeads and magnetic microbeads. They also optimized the conditions for binding affinity of specific capture peptides for spores of two B. anthracis strains including B. anthracis &(number sign)916; Sterne (pXO1-, pXO2-), and B. anthracis Sterne 34F2 (pXO1+, pXO2-). However, further study needs to be investigated for the optimization of binding characteristics of peptide sequence to B. anthracis spores for sensitive and specific detection. In addition, analysis of the Bacillus spore-coat proteins can be beneficial to fine-tune the detection. The sample preparation for detecting dilute B. anthracis spores in various environmental conditions is also another area to be investigated.